I would recommend leaving some space between so light can get in to lower branches, and by having them too close encourages a really moist environment which aphids love (depending on the species your growing. I tried jamming them in nearly next to each other to fit more trees in the same space, but after a while i ended up separating them to 450mm centers, for lighting and for ease of access.
Another thing you will want to consider is leaving a gap between every second the row - enough to kneel or lay down in - I have found myself needing to get right down to the ground level to do some work - just branch/trunk selection, adding fertilizer, wiring.
the 450mm will still be a minimum if your trying to get vertical growth, but if your trying to grow side branching you may want to increase more.
Another thing - I would try using a higher area with good drainage or mound the area up or do it on a slight slope, otherwise I have found the bags being like a sump which collects water in the potting medium compared to the soil around it. this was not a problem for plants which love having wet feet, but for pines and junipers, not ideal to create a swampy environment.
The board in the bag does really help create a good root spread. Good luck